Izindaba - Ukukhiqizwa kwe-vanillin yemvelo kusuka kwizisetshenziswa ezivuselelekayo

I.introduraction

I-Vanillin ingenye yezindawo zokunambitha ezithandwa kakhulu futhi ezisetshenziswa kabanzi emhlabeni. Ngokwesiko, kukhishwe kubhontshisi we-vanilla, okubiza kakhulu futhi abhekana nezinselelo eziphathelene nokusimama nokuhlinzekwa kwamathempeli. Kodwa-ke, nentuthuko e-biotechnology, ikakhulukazi emkhakheni we-microbial biotrancforvation, inkathi entsha yokukhiqizwa kwe-vanillin yemvelo sekuqhamuke. Sisebenzisa ama-microorganisms wokuguqulwa kwemvelo kwezinto zokwakha zemvelo ezihlinzekwe ngezomnotho ze-synthesis ze-vanillin. Le ndlela ayigciniseke kuphela ukukhathazeka kokuqina kodwa futhi inikeza izixazululo ezintsha zomkhakha we-Flavor. Ucwaningo olwenziwe yi-SRM Institute of Science and Technology (SRMITS) luhlinzeke ngokubuyekezwa okuphelele kwezindlela ze-eclectic ekusetshenzisweni kwemvelo kwe-vanillin kanye nezicelo zazo ezifakiwe kanye nezicelo zayo ezahlukahlukene embonini yokudla.

II. Ungayithola kanjani i-vanillin yemvelo kusuka ezinsizakalweni ezivuselelekayo

Ukusetshenziswa kwe-ferulic acid njenge-substrate

I-Ferulic acid, ethathwe emithonjeni efana nelayisi bran bran kanye ne-oat bran, ikhombisa ukufana okuhlelekile ku-vanillin futhi kusebenza njenge-substrate yangaphambili esetshenziswa kabanzi yokukhiqizwa kwe-vanillin. Ama-microorganisms ahlukahlukene afana ne-pseudomonas, i-aspergillus, i-streptomyces, kanye nesikhunta sebesebenzilwe ukukhiqizwa kwe-vanillin kusuka ku-ferulic acid. Okuphawuleka kakhulu, izinhlobo ezinjenge-Amycolatopsis kanye nesikhunta esimhlophe - zikhonjwe njengabazongenela ukhetho lokukhiqiza i-vanillin kusuka ku-ferulic acid. Ucwaningo oluningi luphephe ukukhiqizwa kwe-vanillin kusuka ku-ferulic acid usebenzisa ama-microorganisms, izindlela ze-enzymatic, nezinhlelo ezingekho emthethweni, ziqokomisa ukuguquguquka nokusebenza okuhlukahlukene.

Ukuhlanganiswa kwe-enzymatic kwe-vanillin kusuka ku-Ferulic acid kufaka phakathi i-enzyme feruloyl esterase, ethinta i-hydrolysis ye-ester bond ku-ferulic acid, ukukhulula i-vanillin neminye imikhiqizo ehlobene nemikhiqizo. Ngokuhlola inani elifanele lama-enzyths we-vanillin biosynthtetic asezinhlelweni ezinamaselula, abacwaningi bathuthukise i-escherhia colo strain ethuthukisiwe ekwazi ukuguqula i-ferulic acid (20mm) ibe yi-vanillin (15mm). Ngokwengeziwe, ukusetshenziswa kwe-microbial cell ukungasebenzi kahle kuthathe ukunakwa ngenxa yokuqina kwayo okuhle nokuqina ngaphansi kwezimo ezahlukahlukene. Isu lokuvikelwa kwenoveli lokukhiqizwa kwe-vanillin kusuka ku-ferulic acid selithuthukisiwe, liqede isidingo sama-coenzymes. Le ndlela ifaka phakathi i-coenzyme-uzimele-ezimele deCarboxylase kanye ne-coenzyme-izimele oxygenase ebhekele ukuguqulwa kwe-ferulic acid ku-vanillin. I-Coo-Imfomilization ye-FDC ne-CSO2 inika amandla ukukhiqizwa kuka-2,5 mg we-vanillin kusuka ku-ferulic acid emijikelezweni eyishumi yokuphendula, ukumaka isibonelo sokuphayona ngokukhiqizwa kwe-enzyme nge-enzyme.

Ukusetshenziswa kwe-eugenol / i-isoeugenol njenge-substrate

I-Eugenol ne-Isoeugenol, lapho ifakwa eBioconVersition, ikhiqiza i-vanillin kanye nama-metabolites awo ahlobene nayo, atholakele ukuthi anezicelo ezahlukahlukene kanye nenani lezomnotho elibalulekile. Izifundo eziningana zihlole ukusetshenziswa kwezinto eziguqulwa ngofuzo nokwenziwa ngokwemvelo ukuhlanganisa i-vanillin kusuka eugenol. Amandla e-Eugenol DellaDation aqashelwe kumagciwane ahlukahlukene kanye nesikhunta, kufaka phakathi i-bacillus, ama-pseudomonas, i-aspergillus, kanye ne-rhodococcus, ekhombisa amandla abo ekukhiqizweni kwe-eugenol-desillin okususelwa e-eugenol. Ukusetshenziswa kwe-Eugenol Oxidase (EUGO) njenge-enzyme yokukhiqizwa kwe-vanillin endaweni yezimboni kukhombisile amandla amakhulu. I-EUGO ikhombisa ukuqina kanye nomsebenzi ngaphezulu kwebanga elibanzi le-PH, ngomsebenzi okhulayo we-EUGO futhi wehlisa isikhathi sokuphendula. Ngaphezu kwalokho, ukusetshenziswa kwe-eugo engaguquki kuvumela ukuvuselelwa kwe-biocatalyst kufinyelela ku-18 reaction reaction imijikelezo, okuholela ekukhuphukeni okungaphezulu kwe-12 Ngokufanayo, i-enzyme ye-enzyme engadingekile ingakhuthaza ukuguqulwa kwe-isoeugenol ibe yi-vanillin ngaphandle kokuncika kuma-coenzymes.

Ezinye izimbotshana

Ngaphezu kwe-ferulic acid ne-eugenol, ezinye izinhlanganisela ezifana ne-vanillic acid kanye ne-C6-C3 Phenyllpropanoids zikhonjwe njengezigatshana ezingaba khona ngokukhiqizwa kwe-vanillin. I-VanillIc acid, ekhiqizwa njengomkhiqizo wokuwohloka kwe-lignin noma njengengxenye yokuncintisana ezindaweni ze-metabolic, kubhekwa njengokwandulelwa okuyisisekelo kokukhiqizwa kwe-bio-based vanillin. Ngaphezu kwalokho, ukuhlinzeka ngokuqonda ekusetshenzisweni kwe-C6-C3 Phenyllpropanoids ye-vanillin synthesis kuveza ithuba eliyingqayizivele lokunambitheka okuzinzile futhi okusha okusha.

Ekuphetheni, ukusetshenziswa kwezinsizakusebenza ezivuselelekayo zokukhiqizwa kwe-vanillin zemvelo nge-microbial biotrannationform ukuthuthukiswa komhlaba embonini ye-flavour. Le ndlela inikezela ngenye indlela, ememe indlela yokukhiqizwa kwe-vanillin, ekhuluma nokukhathazeka kokusimama kanye nokunciphisa ukuthembela ezindleleni zokukhishwa kwendabuko. Izicelo ezahlukahlukene kanye nenani lezomnotho le-vanillin embonini yokudla ligcizelela ukubaluleka kokuqhubeka nocwaningo nentuthuko kule ndawo. Intuthuko yesikhathi esizayo emkhakheni we-vanillin wemvelo inamandla okuguqula imboni ye-flavour, enikeza ezinye izindlela ezizinzile nezinobungane ze-flavour innovation. Njengoba siqhubeka nokusebenzisa amandla ezinsizakusebenza ezivuselelekayo kanye nokuthuthuka kwe-biotechnological, ukukhiqizwa kwe-vanillin yemvelo kusuka kuma-substrate ahlukahlukene kuveza indlela ethembisayo yokunambitheka.

III. Yini izinzuzo zokusebenzisa izinsiza ezivuselelekayo ukukhiqiza i-vanillin yemvelo

Imvelo Inemvelo:Kusetshenziswa izinsizakusebenza ezivuselelekayo njengezitshalo kanye nokungcola kwe-biomass ukukhiqiza i-vanillin kunganciphisa isidingo samafutha okugcwala, kunciphise imithelela emibi emvelweni, futhi kunciphise ukuphuma kwegesi ebamba ukushisa.

Ukusimama:Ukusebenzisa izinsizakusebenza ezivuselelekayo kunika amandla okuhlinzekwa kwamandla okuqhubekayo kanye nezinto zokwakha, kusiza ukuvikela izinsiza zemvelo futhi zihlangabezane nezidingo zezizukulwane ezizayo.

Ukuvikelwa Kwezinto Eziphilayo:Ngokusebenzisa okunengqondo kwezinsizakusebenza ezivuselelekayo, izinsizakusebenza zezitshalo zasendle zingavikelwa, ezinomthelela ekuvikelweni kwezinto eziphilayo kanye nokugcinwa kwemali esele yemvelo.

Ikhwalithi Yomkhiqizo:Uma kuqhathaniswa ne-vanillin yokwenziwa, i-vanillin yemvelo ingaba nezinzuzo eziningi ngekhwalithi ye-aroma kanye nezimpawu zemvelo, ezizosiza ukuthuthukisa ikhwalithi yokunambitheka kanye nemikhiqizo yamakha.

Yehlisa ukuncika kumafutha fossil:Ukusetshenziswa kwezinsizakusebenza okuvuselelekayo kusiza ukunciphisa ukuncika kumafutha fossil asread, okuwusizo ekuvikelweni kwamandla kanye nokwehlukahlukana kwamandla. Ngiyethemba imininingwane engenhla ingaphendula imibuzo yakho. Uma udinga idokhumenti lesethenjwa ngesiNgisi, ngicela ungazise ukuze ngikunikeze wena.

IV. Ukugcina

Amandla okusebenzisa izinsizakusebenza ezivuselelekayo ukukhiqiza i-vanillin yemvelo njengenye indlela esimeme nenemvelo enobungane. Le ndlela inesithembiso ekubhekaneni nesidingo esandayo se-vanillin yemvelo ngenkathi inciphisa ukuthembela ezindleleni zokwenziwa zokwenziwa.

I-Natural Vanillin ibamba isikhundla esibalulekile embonini ye-flavour, yenani lephunga layo elicijile nokusetshenziswa okugcwele njenge-ejenti yokunambitha emikhiqizweni ehlukahlukene. Kubalulekile ukugcizelela ukubaluleka kwe-vanillin yemvelo njengezithako ezifunwayo ekudleni, isiphuzo, kanye nezimboni zamakha ngenxa yephrofayili yayo ephakeme yezinzwa kanye nokuthandwa kwabathengi ngezinto ezithandekayo zemvelo.

Ngaphezu kwalokho, insimu yokukhiqizwa kwe-vanillin yemvelo iveza amathuba amakhulu okucwaninga okwengeziwe kanye nentuthuko. Lokhu kufaka phakathi ubuchwepheshe obusha nezindlela ezintsha zokuphakamisa ukuthuthukisa ukusebenza kahle kanye nokusimama kokukhiqiza i-vanillin yemvelo emithotsheni evuselelekayo. Ngokwengeziwe, ukuthuthukiswa kwezindlela zokukhiqiza ezi-scalotable nezingabizi zizodlala indima ebaluleke kakhulu ekukhuliseni ukwamukelwa kwemvelo okusabalele njengendlela eqhubekayo ne-eco-friendly embonini ye-flavour.